To date the process of wound induced hair follicle neogenesis (WIHN) has only been investigated using a candidate-based approach. Here, taking advantage from significant variation of WIHN across murine genetic background, we combine mice from the Collaborative Cross with IF techniques, QTL analysis, and RNA expression datasets to investigate the genetic determinants of WIHN. WIHN varied significantly across 37 murine strains according to number of regenerated hairs. Genome wide linkage identified a region where Sema6a appeared as a potential candidate, as it is also a signature gene of the developing dermal condensate. Sema6a was differentially expressed in the skin of strains with High versus low levels of WIHN. Moreover, in single cell RNA expression analyses Sema6a was expressed in Shh-active wound fibroblasts and largely reciprocally expressed with plexin receptors suggesting that it may participate in dermal condensation during WIHN.